Kai Hsia
TEL: +886- 928809129
E-mail: xkay1008@yahoo.com.tw
hkay1008@gmail.com
Education
1989-1993 Beijing University, Beijing, P. R. C
MD Dept. of Medicine
2011-2012 University of Liverpool. The UK
MRes Biomedical Science and Translational medicine
Employment
2012- now Taipei Veterans General Hospital, Taiwan
Quality director Cryopreserved heart valve and vessel tissue bank
2009- 2011 Taipei Veterans General Hospital, Taiwan
Technician Cryopreserved heart valve and vessel tissue bank
2007-2009 HealthBanks Biotech. Co., Ltd, Taipei, Taiwan
Senior Researcher R & D Department
Stem Cell Research & Protein Vaccine Lab
Stem Cell bank
2001-2006 Gene Research Lab. Co., Ltd, Taipei, Taiwan
Asistant Manager R & D Department
1999 - 2001 Veterans General Hospital, Taipei, Taiwan
Research Assistant Dept. of Surgery
Training Course Certificate
CTBS by AATB (American Association of Tissue Bank)
ISO17025
cGMP
GLP
Professionalism
TISSUE BANK--- Heart valve and cardiovascular processing (processing, preservation, quarantine and storage, distribution), Quality control.
MOL BIOL---DNA or RNA purification, PCR, RT-PCR, DNA cloning, Transformation, Transfection,
BIOCHEM---ELISA, Western blotting, Protein expression (in prokaryote or eukaryote system) and purification(Gel filtration, rProtein A, Sepharose, ion exchange, affinity, HIC, etc.), Protein refolding
CELL---Basic cell culture skills, Human PBMC separation and proliferation assay, FACScan flow cytometry(cell surface marker stain , DNA stain, intercellular cytokine stain, stem cell QC, Apoptosis test, allergen test of peripheral blood, etc.), polyclone and Monoclonal antibody preparation, MTT assay, drug screenings, apoptosis test, angiogenesis test, MSC reseach. Stem cell culture and differentiations .
Animal's test and Immunology experiment -IHC stain, ELISA development, protein chip, etc),