探討Sphingosine-1-Phosphate在構建以幹細胞臍靜脈為基礎的組織工程小口徑血管過程中的作用及其作用機理

 


 



Kai Hsia

 TEL: +886- 928809129
      E-mail: xkay1008@yahoo.com.tw 
hkay1008@gmail.com

 

Education

1989-1993                             Beijing University, Beijing, P. R. C   
MD                                        Dept. of Medicine

2011-2012               University of Liverpool. The UK
MRes                     Biomedical Science and Translational medicine 

Employment
2012- now           Taipei Veterans General Hospital, Taiwan
Quality director         Cryopreserved heart valve and vessel tissue bank

2009- 2011          Taipei Veterans General Hospital, Taiwan
Technician             Cryopreserved heart valve and vessel tissue bank

2007-2009                 HealthBanks Biotech. Co., Ltd, Taipei, Taiwan
Senior Researcher                  R & D Department
Stem Cell Research &        Protein Vaccine Lab
Stem Cell bank

2001-2006                    Gene Research Lab. Co., Ltd, Taipei, Taiwan
Asistant Manager                       R & D Department

 

1999 - 2001                   Veterans General Hospital, Taipei, Taiwan
Research Assistant             Dept. of Surgery

 

Training Course Certificate
CTBS by AATB (American Association of Tissue Bank)
ISO17025
cGMP
GLP

Professionalism
TISSUE BANK--- Heart valve and cardiovascular processing (processing, preservation, quarantine and storage, distribution), Quality control.

MOL BIOL---DNA or RNA purification, PCR, RT-PCR, DNA cloning, Transformation, Transfection,

BIOCHEM---ELISA, Western blotting, Protein expression (in prokaryote or eukaryote system) and purification(Gel filtration, rProtein A, Sepharose, ion exchange,  affinity, HIC, etc.), Protein refolding

CELL---Basic cell culture skills, Human PBMC separation and proliferation assay, FACScan flow cytometry(cell surface marker stain , DNA stain, intercellular cytokine stain, stem cell QC, Apoptosis test, allergen test of peripheral blood, etc.), polyclone and Monoclonal antibody preparation, MTT assay, drug screenings, apoptosis test, angiogenesis test, MSC reseach. Stem cell culture and differentiations .

Animal's test and Immunology experiment -IHC stain, ELISA development, protein chip, etc),

 








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電話 (TEL) - (02)-33662499

地址 (Address) - 106 台北市羅斯福路四段一號 國立台灣大學生命科學系 504 室

. 建立日期:2007年7月
. 最後更新:2017年10月

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